Journal: Nature Communications

Article Title: Defect engineering of layered double hydroxide nanosheets as inorganic photosensitizers for NIR-III photodynamic cancer therapy

doi: 10.1038/s41467-022-31106-9

Figure Lengend Snippet: a Biodistribution of DR-CoMo-LDH-PEG in mice by monitoring the Co concentration at various time points post-injection. Data are presented as mean values ± s.d. ( n = 3). Statistical analysis was performed via one-way ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001. b Tumor growth curves of mice after various treatments. Data are presented as mean values ± s.d. ( n = 6). Statistical analysis was performed via one-way ANOVA. ***p < 0.001. c Representative photographs of mice with treatments at various time points and d corresponding average weight of tumors taken on Day 16. Data are presented as mean values ± s.d. ( n = 6). Statistical analysis was performed via one-way ANOVA. ***p < 0.001. e Body weight change of 4T1 tumor-bearing mice after different treatments. Data are presented as mean values ± s.d. ( n = 6). f HIF-1α, g DHE, and h H&E, Ki-67, TUNEL and CD31 staining assay of tumor slices from various groups of mice after 16 days of treatment: 1) PBS, 2) 1567 nm laser (0.5 W cm −2 for 6 min), 3) DR-CoMo-LDH-PEG, 4) DR-CoMo-LDH-PEG + 1567 nm laser. Each experiment was repeated three times with similar results. Source data are provided as a Source Data file.

Article Snippet: The tumor slices were stained with primary antibodies (against HIF-1α (GB13031-1, Servicebio, China, 1:200 dilution)) at 4 °C overnight, followed by phalloidin at 25 °C for 1 h. Images were captured via CLSM.

Techniques: Concentration Assay, Injection, TUNEL Assay, Staining

Journal: International Journal of Biological Sciences

Article Title: Over-activation of TRPM2 ion channel accelerates blood-spinal cord barrier destruction in diabetes combined with spinal cord injury rat

doi: 10.7150/ijbs.80672

Figure Lengend Snippet: Diabetes impedes neovascularization level in spinal cord of SCI rat. (A and B) Representative western blotting results and quantitative analysis of HIF-1α, ANG1 and VEGF in spinal cord at 3 days after SCI. (C and D) Representative western blotting results and quantitative analysis of HIF-1α, ANG1 and VEGF in HUVECs. (E) Co-staining of HIF-1α (green) and CD31 (red) in spinal cord at 3 days after SCI, Scale bar = 10 μm. (F) Co-staining of ANG1 (green) and CD31 (red) in spinal cord at 3 days after SCI, Scale bar = 10 μm. (G) The tube formation ability of HUVECs, Scale bar = 200μm. (H) Quantification of tube length from tube formation assay. n≥3, *P<0.05, **P<0.01, ***P<0.001.

Article Snippet: Then, the membranes were blocked with 5% skimmed milk in TBST for 2 h at room temperature, and incubated with the following primary antibodies at 4°C overnight: TRPM2 (1:1000, NB500-242), p-CaMKII (1:1000, sc-32289), CaMKII (1:1000, sc-5306), eNOS (1:1000, sc-376751), NOX2 (1:1000, ab129068), HIF-1α (1:1000, ZEN BIO, 340462), ANG1 (1:5000, ab183701), VEGF (1:1000, sc-7269), Cleaved(C)-caspase3 (1:1000, 9664S), β-catenin (1:1000, ab32572), ZO-1 (1:1000, AF5145), Claudin-5 (1:1000, AF5216), occluding (1:1000, DF7504), P120 (1:1000, AF4684).

Techniques: Western Blot, Staining, Tube Formation Assay

Journal: International Journal of Biological Sciences

Article Title: Over-activation of TRPM2 ion channel accelerates blood-spinal cord barrier destruction in diabetes combined with spinal cord injury rat

doi: 10.7150/ijbs.80672

Figure Lengend Snippet: TRPM2 inhibition improves angiogenesis level under diabetes combined with SCI condition. (A and B) Representative western blotting results and quantitative analysis of HIF-1α, ANG1 and VEGF in spinal cord of rat at 3 days after SCI. (C and D) Representative western blotting results and quantitative analysis of HIF-1α, ANG1 and VEGF in HUVECs. (E) Co-staining of HIF-1α (green) and CD31 (red) in spinal cord at 3 days after SCI, Scale bar = 10 μm. (F) Co-staining of ANG1 (green) and CD31 (red) in spinal cord at 3 days after SCI, Scale bar = 10 μm. (G) The tube formation ability of HUVECs, Scale bar = 200 μm. (H) Quantification of tube length from tube formation assay. n≥3, *P<0.05, **P<0.01, ***P<0.001.

Article Snippet: Then, the membranes were blocked with 5% skimmed milk in TBST for 2 h at room temperature, and incubated with the following primary antibodies at 4°C overnight: TRPM2 (1:1000, NB500-242), p-CaMKII (1:1000, sc-32289), CaMKII (1:1000, sc-5306), eNOS (1:1000, sc-376751), NOX2 (1:1000, ab129068), HIF-1α (1:1000, ZEN BIO, 340462), ANG1 (1:5000, ab183701), VEGF (1:1000, sc-7269), Cleaved(C)-caspase3 (1:1000, 9664S), β-catenin (1:1000, ab32572), ZO-1 (1:1000, AF5145), Claudin-5 (1:1000, AF5216), occluding (1:1000, DF7504), P120 (1:1000, AF4684).

Techniques: Inhibition, Western Blot, Staining, Tube Formation Assay

Journal:

Article Title: Stage Specific Inhibitory Effects and Associated Mechanisms of Silibinin on Tumor Progression and Metastasis in TRAMP Model

doi: 10.1158/0008-5472.CAN-08-1332

Figure Lengend Snippet: Stage specific effect of silibinin feeding on angiogenesis and pro-angiogenic markers in TRAMP prostate. (A, left) Effect of Silibinin feeding on intraductal MVD as inferred by IHC staining for the expression of PECAM-1/CD-31. IHC staining was based on DAB staining as detailed in “Materials and Methods”. Quantification of PECAM-1/CD-31-positive cells for determination of MVD is shown as mean and ± SEM (error bars) in each group. MVD was calculated as the number of positive cells × 100 / total number of cells counted under ×40 magnifications in 5 selected areas in each sample. (A, right) Stage specific effect of silibinin feeding on VEGF expression in TRAMP mice prostate as determined by WB analysis. (B) Stage specific effect of silibinin feeding on the expression levels of VEGF-R1 and VEGF-R2 in TRAMP mice prostate as determined by WB analysis. (C-D) Stage specific effect of silibinin feeding on the expression levels of HIF-1α and iNOS in TRAMP mice prostate as determined by IHC/WB analysis. Randomly, four prostate tissue samples from individual mice were selected from each group for WB analysis as detailed in “Materials and Methods”. Reactive protein bands were visualized by enhanced chemiluminescence detection system, and membrane were stripped and probed with β-actin as loading control. Densitometric analysis of band intensity for each protein was adjusted with β-actin (blots not shown). The results were reported as mean and ± SEM (error bars) of the four bands from individual mouse prostate in each group based on the relative densities compared to the 4-12 positive control group. Representative blots of two prostate samples from each group are shown. Difference between the positive control groups was determined by one-way ANOVA followed by Tukey-test for multiple comparisons, and values are mentioned only in the “Results section”. The difference between the positive controls versus the respective silibinin-fed group was analyzed by unpaired two-tailed Student’s t-test. P values <0.05 were considered significant. *, P<0.001; #, P<0.01; ψ P<0.02, $, P<0.05. Control, positive control (TRAMP mice); Sb, silibinin; MVD, microvessel density; platelet endothelial cell adhesion molecule-1 (PECAM-1/CD-31); VEGF, vascular endothelial growth factor; VEGF-R1, VEGF receptor-1; VEGF-R2, VEGF receptor-2; HIF-1α , hypoxia-inducible factor-1α; iNOS, inducible nitric oxide synthase; WB, western blot; IHC, immunohistochemical.

Article Snippet: Primary antibodies used were against PCNA (Dako); PECAM-1/CD-31, VEGF (Santa Cruz Biotechnology); SV40 large T antigen (BD Pharmingen); HIF-1α (Novartis) and iNOS (Abcam).

Techniques: Immunohistochemistry, Expressing, Staining, Membrane, Control, Positive Control, Two Tailed Test, Western Blot, Immunohistochemical staining